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Related Concept Videos

Protein Networks02:26

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Proteomics01:33

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Ribosome Profiling02:24

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Protein-protein Interfaces02:04

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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Related Experiment Video

Updated: Jul 16, 2025

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
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2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

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Activity-based protein profiling: A graphical review.

Exequiel O J Porta1, Patrick G Steel1

  • 1Department of Chemistry, Durham University, Durham, DH1 3LE, United Kingdom.

Current Research in Pharmacology and Drug Discovery
|September 11, 2023
PubMed
Summary
This summary is machine-generated.

Activity-based protein profiling (ABPP) uses chemical probes to study protein function. This review highlights ABPP

Keywords:
Activity-based probesActivity-based protein profilingAffinity-based probesChemical probesChemoproteomicsDrug discoveryMass spectrometryTarget discovery

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Area of Science:

  • Chemoproteomics
  • Chemical Biology
  • Proteomics

Background:

  • Activity-based protein profiling (ABPP) is a chemoproteomic technology.
  • It uses small chemical probes to interrogate protein function.
  • ABPP has been used for nearly 25 years.

Purpose of the Study:

  • To provide an overview of the evolution of ABPP strategies.
  • To highlight the versatility of ABPP.
  • To showcase successful applications of ABPP in drug discovery.

Main Methods:

  • Review of ABPP strategies.
  • Graphical representation of ABPP evolution.
  • Selected examples of ABPP applications.

Main Results:

  • ABPP has rapidly evolved over the past 25 years.
  • ABPP is a versatile tool for drug discovery.
  • ABPP aids in developing selective inhibitors, discovering targets, and illuminating proteins.

Conclusions:

  • ABPP is a powerful technology for interrogating protein function.
  • Its applications span drug discovery, target identification, and biological studies.
  • The continued evolution of ABPP promises further advancements.