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Updated: Jul 16, 2025
Oncogene Expression Analysis with Alterations in pH in a Pancreatic Ductal Cell Line
Published on: April 11, 2025
A pan-cancer analysis of pituitary tumor-transforming 3, pseudogene
Jie Li1, Saima Naz Shaikh2, Arsalan Ahmed Uqaili2
1The Second Affiliated Hospital of Hainan Medical University Health Management Center Haikou 570311, Hainan, China.
Background:
Although evidence regarding pituitary tumor-transforming 3, pseudogene (PTTG3P) involvement in human cancers has been acquired via human and animal model-based molecular studies, there is a lack of pan-cancer analysis of this gene in human tumors.
Methods:
Tumor-causing effects of PTTG3P in 24 human tumors were explored using The Cancer Genome Atlas (TCGA) datasets from different bioinformatics databases and applying in silico tools such as The University of ALabama at Birmingham CANcer (UALCAN), Human Protein Atlas (HPA), Kaplan Meier (KM) plotter, cBioPortal, Search Tool for the Retrieval of Interacting Genes/Proteins (STRING), Cytoscape, Database for Annotation, Visualization, and Integrated Discovery (DAVID), Tumor IMmune Estimation Resource (TIMER), and Comparative Toxicogenomics Database (CTD). Then, via in vitro experiments, including RNA sequencing (RNA-seq) and targeted bisulfite sequencing (bisulfite-seq), expression and promoter methylation levels of PTTG3P were verified in cell lines.
Results:
The PTTG3P expression was overexpressed across 23 malignancies and its overexpression was further found significantly effecting the overall survival (OS) durations of the esophageal carcinoma (ESCA) and head and neck cancer (HNSC) patients. This important information helps us to understand that PTTG3P plays a significant role in the development and progression of ESCA and HNSC. As for PTTG3P functional mechanisms, this gene along with its other binding partners was significantly concentrated in "Oocyte meiosis", "Cell cycle", "Ubiquitin mediated proteolysis", and "Progesterone-mediated oocyte maturation". Moreover, ESCA and HNSC tissues having the higher expression of PTTG3P were found to have lower promoter methylation levels of PTTG3P and higher CD8+ T immune cells level. Additionally, PTTG3P expression-regulatory drugs were also explored in the current manuscript for designing appropriate treatment strategies for ESCA and HNSC with respect to PTTG3P expression.
Conclusion:
Our pan-cancer based findings provided a comprehensive account of the oncogenic role and utilization of PTTG3P as a novel molecular biomarker of ESCA and HNSC.
Insights
Pituitary tumor-transforming 3, pseudogene (PTTG3P) is overexpressed in 23 cancers, impacting survival in esophageal carcinoma and head and neck cancer. PTTG3P serves as a potential biomarker for these cancers.
Area of Science:
- Oncology
- Genomics
- Bioinformatics
Background:
- Pituitary tumor-transforming 3, pseudogene (PTTG3P) involvement in human cancers is understudied.
- A comprehensive pan-cancer analysis of PTTG3P in human tumors is lacking.
Purpose of the Study:
- To investigate the tumor-causing effects of PTTG3P across 24 human tumors.
- To explore the potential of PTTG3P as a molecular biomarker in cancer.
Main Methods:
- Utilized The Cancer Genome Atlas (TCGA) datasets and various bioinformatics tools for in silico analysis.
- Performed in vitro experiments including RNA sequencing (RNA-seq) and bisulfite sequencing to validate PTTG3P expression and methylation levels.
Main Results:
- PTTG3P was overexpressed in 23 malignancies, significantly affecting overall survival in esophageal carcinoma (ESCA) and head and neck cancer (HNSC).
- PTTG3P functions were linked to "Oocyte meiosis", "Cell cycle", and "Ubiquitin mediated proteolysis".
- Higher PTTG3P expression in ESCA and HNSC correlated with lower promoter methylation and increased CD8+ T cells, with potential therapeutic drugs identified.
Conclusions:
- PTTG3P demonstrates an oncogenic role across multiple cancers.
- PTTG3P shows promise as a novel molecular biomarker for esophageal carcinoma and head and neck cancer.
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