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Related Concept Videos

Proteomics01:33

Proteomics

7.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.4K

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Related Experiment Video

Updated: Jul 16, 2025

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
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Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

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Protein Coronas on Functionalized Nanoparticles Enable Quantitative and Precise Large-Scale Deep Plasma Proteomics.

Ting Huang1, Jian Wang1, Alexey Stukalov1

  • 1Seer, Inc., Redwood City, CA, 94065 USA.

Biorxiv : the Preprint Server for Biology
|September 11, 2023
PubMed
Summary

Automated nanoparticle (NP) workflows enable deep plasma proteomics, identifying over 6,000 proteins. This high-throughput method enhances biomarker discovery and quantitative analysis for diagnostics.

Keywords:
LC-MSProteomicsclinical proteomicscohortnanoparticlesplasmaquantification

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Area of Science:

  • Proteomics
  • Biomarker Discovery
  • Mass Spectrometry

Background:

  • Plasma proteome characterization is challenging due to wide dynamic range and diverse proteoforms.
  • Automated nanoparticle (NP) protein corona workflows compress dynamic range for mass spectrometry (MS).
  • This enhances depth, scalability, and comprehensiveness of quantitative MS-based methods for diagnostics.

Conclusions:

  • Automated NP workflows facilitate high-throughput, deep, and quantitative plasma proteomics.
  • The method provides sufficient statistical power for discovering novel biomarker signatures.
  • Peptide-level resolution is achieved in comprehensive plasma proteome investigations.