Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.1K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.1K
Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response01:15

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

87
Circadian rhythms are cyclic changes that are crucial in plasma drug concentrations. Various standard circadian parameters, including core body temperature, heart rate, and other cardiovascular factors, directly impact disease states and the therapeutic response to drug therapy.
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...
87
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
Factors Affecting Drug Biotransformation: Biological01:19

Factors Affecting Drug Biotransformation: Biological

177
Biological factors significantly impact drug metabolism, influencing drug clearance, efficacy, and potential toxicity.
Species differences: Variations in enzyme systems across species can cause disparities in drug metabolism. For instance, humans may metabolize certain drugs faster than rodents, altering therapeutic effects.
Strain differences: Genetic variations within a species can result in differing enzyme activity, impacting drug response and toxicity. For example, some mouse strains may...
177
Global Regulatory Systems01:28

Global Regulatory Systems

44
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
44
Biological Clocks and Seasonal Responses02:45

Biological Clocks and Seasonal Responses

34.7K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
34.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stress Responsive bZIP Transcription Factors ATF4 and BACH1 Cooperate With MAF-Family bZIP Protein NRL to Fine-Tune Rod Photoreceptor Gene Expression.

Investigative ophthalmology & visual science·2026
Same author

Downregulation of RORα by alcohol promotes TGFβ and α-SMA expression in mouse lung fibroblasts.

Frontiers in medicine·2026
Same author

Development of a Direct Sampling Module for Compact Capillary Liquid Chromatography.

Journal of separation science·2025
Same author

<i>Bmal1</i> deletion alters mitochondrial microstructure and function in mouse cone photoreceptors.

iScience·2025
Same author

When no answer is better than a wrong answer: A causal perspective on batch effects.

Imaging neuroscience (Cambridge, Mass.)·2025
Same author

From Pineal to Biological Rhythms Through Melatonin.

Journal of pineal research·2025

Related Experiment Video

Updated: Jul 16, 2025

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
11:56

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

9.8K

Environmental circadian disruption re-programs liver circadian gene expression.

Hao A Duong1,2, Kenkichi Baba1,2, Jason P DeBruyne1,2

  • 1Department of Pharmacology and Toxicology, Morehouse School of Medicine, Atlanta GA 30310.

Biorxiv : the Preprint Server for Biology
|September 11, 2023
PubMed
Summary

Environmental-circadian disruption (ECD) profoundly alters liver circadian gene expression, impacting metabolism. Most molecular rhythms are re-written by ECD, with effects persisting even after recovery.

More Related Videos

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System
07:34

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System

Published on: December 16, 2022

2.3K
Circadian Entrainment of Drosophila Melanogaster
07:12

Circadian Entrainment of Drosophila Melanogaster

Published on: June 3, 2020

4.4K

Related Experiment Videos

Last Updated: Jul 16, 2025

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
11:56

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

9.8K
Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System
07:34

Manipulation of Rhythmic Food Intake in Mice Using a Custom-Made Feeding System

Published on: December 16, 2022

2.3K
Circadian Entrainment of Drosophila Melanogaster
07:12

Circadian Entrainment of Drosophila Melanogaster

Published on: June 3, 2020

4.4K

Area of Science:

  • Chronobiology
  • Molecular Biology
  • Metabolomics

Background:

  • Circadian gene expression is crucial for the conserved biological clock.
  • Environmental-circadian disruption (ECD) from shiftwork and jetlag affects millions but its molecular impact is unclear.
  • Understanding ECD's effects on liver circadian rhythms is vital for public health.

Approach:

  • Comprehensive analysis of liver circadian gene expression under normal and ECD conditions.
  • Investigated transcriptome, whole-cell proteome, and nuclear proteome.
  • Examined changes in cis-regulatory elements, RNA processing, and protein trafficking.

Key Points:

  • Post-transcriptional and post-translational modifications significantly shape circadian proteomes.
  • ECD re-writes the rhythmicity of 64% of the transcriptome, 98% of the whole-cell proteome, and 95% of the nuclear proteome.
  • Metabolic regulation of fat and carbohydrates is impaired by ECD.

Conclusions:

  • ECD drastically alters liver molecular rhythms, affecting fundamental biological processes.
  • The observed changes in gene expression and protein rhythmicity are linked to altered regulatory mechanisms.
  • Impaired metabolic regulation persists post-ECD, highlighting long-term health consequences.