Hsa_ circ_0006867 regulates ox-LDL-induced endothelial injury via the miR-499a-3p/ADAM10 axis

Area of Science:

• Molecular Biology
• Cardiovascular Research
• Genetics

Background:

• Circular RNAs (circRNAs) are implicated in various disease pathologies.
• The specific role of circRNAs in atherosclerosis development requires further elucidation.
• Endothelial dysfunction is a key event in atherosclerosis pathogenesis.

Purpose of the Study:

• To investigate the mechanism of circRNAs in atherosclerosis.
• To identify specific circRNAs involved in oxidized low-density lipoprotein (ox-LDL)-induced endothelial injury.
• To explore the therapeutic potential of identified circRNAs in atherosclerosis.

Main Methods:

• Human umbilical vein endothelial cells (HUVECs) were treated with ox-LDL to model atherosclerosis.
• Expression levels of circRNAs were analyzed in treated endothelial cells.
• The interaction between circ_0006867, miR-499a-3p, and ADAM10 was investigated using molecular assays.

Main Results:

• Hsa_circ_0006867 (circ_0006867) expression was significantly upregulated in ox-LDL-treated HUVECs.
• Circ_0006867 functioned as a molecular sponge for miR-499a-3p, regulating its availability.
• This interaction modulated the expression of the downstream target gene ADAM10, affecting endothelial cell apoptosis and migration.

Conclusions:

• Circ_0006867 plays a critical role in ox-LDL-induced endothelial injury through the circ_0006867/miR-499a-3p/ADAM10 axis.
• Circ_0006867 represents a potential therapeutic target for atherosclerosis.
• Understanding this regulatory axis provides insights into endothelial cell function in cardiovascular disease.