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Updated: Jul 16, 2025
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Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay
Published on: October 4, 2024
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Expression and function of CCN2-derived circRNAs in chondrocytes
Soma Kato1,2, Kazumi Kawata1, Takashi Nishida1
1Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-Cho, Kita-Ku, Okayama, 700-8525, Japan.
Journal of Cell Communication and Signaling
|September 11, 2023
Summary
Novel circular RNAs (circRNAs) derived from Cellular Communication Network Factor 2 (CCN2) are present in chondrocytes and influence chondrogenic differentiation, impacting aggrecan and proteoglycan synthesis.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Cellular Communication Network Factor 2 (CCN2) is crucial for endochondral ossification and cartilage repair.
- Circular RNAs (circRNAs) regulate gene expression and are known to derive from CCN2 in some cell types.
- The presence and function of CCN2-derived circRNAs in chondrocytes are currently unknown.
Purpose of the Study:
- To investigate the expression and function of CCN2-derived circRNAs in chondrocytes.
- To identify novel CCN2-derived circRNAs in human chondrocytic cells.
- To elucidate the role of these circRNAs in chondrogenic differentiation.
Main Methods:
- RT-PCR analysis to detect and amplify CCN2-derived circRNAs in human chondrocytic HCS-2/8 cells.
- Nucleotide sequencing to identify novel circRNAs.
- Confirmation of circRNA expression in murine ATDC5 cells during chondrocytic differentiation.
- siRNA-mediated knockdown of a specific CCN2-derived circRNA in HCS-2/8 cells.
Main Results:
- Novel CCN2-derived circRNAs, distinct from previously known ones, were identified in HCS-2/8 cells.
- Expression of several CCN2-derived circRNAs was confirmed in ATDC5 cells and varied with chondrocytic differentiation.
- Knockdown of a CCN2-derived circRNA led to a significant decrease in aggrecan mRNA expression and proteoglycan synthesis.
Conclusions:
- CCN2-derived circRNAs are expressed in chondrocytes.
- These circRNAs play a significant role in chondrogenic differentiation.
- CCN2-derived circRNAs are potential regulators of cartilage formation and regeneration.
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