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Tissue Transplantation

Tissue Transplantation

Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...

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Updated: Jul 16, 2025

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Published on: February 10, 2018

Hepatocyte transplantation: The progress and the challenges.

Zhen Sun^1,2, Xiang Yuan¹, Jingqi Wu¹

¹State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

Hepatology Communications

|September 11, 2023

Summary

Hepatocyte transplantation shows promise for liver diseases but faces challenges in cell availability, engraftment, and immune rejection. Advances in cell expansion and preconditioning improve outcomes, yet further research is needed for clinical success.

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Area of Science:

Hepatology
Cell Biology
Immunology

Background:

Hepatocyte transplantation is a potential therapy for liver diseases like metabolic disorders and acute liver failure.
Clinical application is limited by challenges in obtaining quality hepatocytes, ensuring successful engraftment, and managing immune rejection.

Purpose of the Study:

To review recent advancements in hepatocyte transplantation for liver diseases.
To identify key hurdles and potential solutions for improving therapeutic efficacy.

Main Methods:

In vitro expansion of primary human hepatocytes via dedifferentiation.
Enhancement of transplantation efficiency using hepatic irradiation and mitogenic stimuli.
Development of immunosuppressive strategies involving glucocorticoids and T-cell co-stimulation inhibitors.

Main Results:

In vitro cell expansion techniques have advanced, increasing the availability of hepatocytes.
Preconditioning regimens involving irradiation and mitogenic stimuli improve transplant efficiency.
Immunosuppressive strategies are being refined to prevent both innate and adaptive immune rejection.

Conclusions:

Significant progress has been made in hepatocyte transplantation, particularly in cell sourcing and transplantation efficiency.
Further research is essential to optimize in vitro expansion, preconditioning, and immunosuppression for clinical translation.
Ex vivo gene correction for autologous hepatocyte transplantation presents a future direction.