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H.O.S.T.: Hemoglobin microbubble-based Oxidative stress Sensing Technology.

Antra Ganguly1, Sugandha Chaudhary1, Shashank R Sirsi1

  • 1Department of Bioengineering, The University of Texas at Dallas, Richardson, TX, 75080, USA.

Scientific Reports
|September 11, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces H.O.S.T., a novel hemoglobin microbubble biosensor for detecting Hydrogen peroxide (H2O2). The sensor offers improved sensitivity and a lower detection limit for oxidative stress and cancer diagnostics.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Biosensors

Background:

  • Hydrogen peroxide (H2O2) is a key biomarker for oxidative stress and cancer.
  • Existing H2O2 biosensors often face limitations in dynamic range, detection limits, and resolution.
  • Novel sensor development is crucial for accurate and sensitive H2O2 detection in diagnostics.

Purpose of the Study:

  • To develop a novel hemoglobin microbubble-based electrochemical biosensor (H.O.S.T.) for label-free H2O2 detection.
  • To enhance sensor performance, including dynamic range, detection limit, and resolution.
  • To evaluate the biosensor's potential for oxidative stress and cancer diagnostics.

Main Methods:

  • Sonochemical preparation of hemoglobin microbubbles (HbMbs) as capture probes.
  • Characterization of HbMbs using Coulter Counter analysis and Brightfield microscopy.
  • Electrochemical characterization and calibration using EIS, CV, and SWV.
  • Specificity assessment via cross-reactivity studies with ascorbic acid and glucose.
  • Validation in cancerous and non-cancerous cell lysates.

Main Results:

  • The developed H.O.S.T. biosensor demonstrated an extended dynamic range, lower detection limit, and enhanced resolution compared to native hemoglobin sensors.
  • High linearity (R² > 0.95) was achieved during electrochemical calibration.
  • Excellent specificity for H2O2 was confirmed, with minimal cross-reactivity.
  • The biosensor showed good agreement with a commercial H2O2 detection kit.
  • Significant H2O2 detection was achieved in both cancerous and non-cancerous cell lysates.
  • A high affinity for H2O2 (Michaelis Menten constant of 19.44 µM) was observed, attributed to the unique HbMb design.

Conclusions:

  • The novel hemoglobin microbubble-based electrochemical biosensor (H.O.S.T.) is effective for label-free H2O2 detection.
  • The unique HbMb design significantly enhances sensor performance and H2O2 affinity.
  • The H.O.S.T. biosensor shows promising diagnostic potential for oxidative stress and cancer applications.