[Establishment of a spontaneously transformed human fetal lung cell line and its biological characteristics]

A spontaneously transformed human fetal lung cell line (HFLT) was derived from a human fetal lung diploid fibroblast cell line (2BS) by continuous culture. The biological characteristics of this cell line were studied and compared with those of 2BS cells. Accompanying the morphological alteration, the growth rate of the transformed cell was accelerated and the maximum cell density was increased. The anchorage-independent growth was shown by its ability to form colonies in soft agar. The collagen synthesis phenotype of 2BS cells was changed. In addition, the increased chromosome number and the nodule formation after heterotransplantation were pathognomonic of malignant transformation.