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Near-infrared Fluorescent Probes with Long-acting Cyclic Monitoring and Effectively Eliminating Peroxynitrite
Jiezhen Zhuo1, Jin Hui1, Haijun Chi1
1School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Zhong Road, Anshan, 114051, P. R. China.
Chemistry, an Asian Journal
|September 12, 2023
Summary
New fluorescent probes can monitor and eliminate peroxynitrite (ONOO-) in real-time. These probes offer long-acting cyclic monitoring and in-situ elimination, maintaining physiological balance.
Area of Science:
- Chemical sensing
- Biomedical engineering
- Fluorescent probes
Background:
- Peroxynitrite (ONOO-) is a reactive nitrogen species implicated in various physiological and pathological processes.
- Accurate monitoring and regulation of ONOO- levels are crucial for understanding cellular functions and disease mechanisms.
Purpose of the Study:
- To develop novel fluorescent probes for real-time, cyclic monitoring of peroxynitrite (ONOO-).
- To investigate the probes' capability for in-situ elimination of excess ONOO-.
- To assess the probes' potential for maintaining physiological ONOO- balance in biological systems.
Main Methods:
- Synthesis of two novel through-bond energy transfer fluorescent probes based on a dihydroxyl naphthyl-pyrenyl conjugated system.
- Characterization of probe properties, including large Stokes shifts and fluorescence response to varying ONOO- concentrations under physiological conditions.
- Evaluation of probe reversibility, real-time monitoring capacity, and ONOO- decomposition acceleration.
- In-situ biological studies in cells to assess probe performance in eliminating and sensing both exogenous and endogenous ONOO-.
Main Results:
- The synthesized probes exhibit large Stokes shifts (230 or 280 nm) and rapid fluorescence changes (at 620 or 652 nm) in response to ONOO-.
- Probes demonstrated good reversibility, enabling real-time monitoring of ONOO- concentration dynamics.
- Addition of probes significantly accelerated ONOO- decomposition, indicating an elimination function.
- Biological studies confirmed effective and reversible in-situ elimination of both exogenous and endogenous ONOO- in cells.
Conclusions:
- The developed fluorescent probes offer a unique multifunctional system for real-time detection, long-acting cyclic monitoring, and in-situ elimination of peroxynitrite (ONOO-).
- This technology provides a novel approach to maintain normal physiological ONOO- balance in organisms.
- Represents the first probe system capable of achieving simultaneous sensing and elimination for dynamic ONOO- regulation.
