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Hydrogen Ion Capturing Hydrogel Microspheres for Reversing Inflammaging.

Dandan Zheng1,2, Wei Chen1,3, Tongtong Chen1

  • 1Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, P. R. China.

Advanced Materials (Deerfield Beach, Fla.)
|September 12, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a hydrogel microsphere that neutralizes acidity and releases therapeutic agents to combat inflammaging. This approach inhibits the NLRP3 pathway, promoting tissue regeneration in aging-related conditions.

Keywords:
ECM synthesisIVDD (intervertebral disc degeneration)biomineralizationhydrogel microspheresinflammagingmicrofluidics

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Aging Research

Background:

  • Inflammaging, linked to aging diseases, is exacerbated by acidic microenvironments.
  • Maintaining extracellular pH balance is crucial for mitigating inflammaging and repairing tissue damage.

Purpose of the Study:

  • To develop a hydrogen ion-capturing hydrogel microsphere (GMNP) for inflammaging.
  • To block the NLRP3 inflammasome cascade axis in aging tissues.

Main Methods:

  • Biomimetic mineralization and microfluidic technology to create GMNPs.
  • GMNPs incorporate mineralized transforming growth factor-β (TGF-β) and catalase (CAT) nanoparticles.
  • Neutralization of acidic microenvironment via calcium carbonate mineralization layer and release of TGF-β and CAT.

Main Results:

  • GMNP effectively neutralized excess hydrogen ions, creating a balanced microenvironment.
  • In vitro studies showed GMNP suppressed the TXNIP/NLRP3/IL-1β axis and enhanced extracellular matrix (ECM) synthesis.
  • In vivo studies demonstrated GMNP inhibited inflammaging and promoted intervertebral disc regeneration.

Conclusions:

  • GMNP effectively reverses inflammaging by targeting the NLRP3 pathway.
  • The developed hydrogel microsphere provides a sustainable niche for inhibiting inflammaging and promoting tissue repair.
  • This technology offers a novel strategy for treating aging-related tissue degeneration.