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Updated: Jul 16, 2025

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
Published on: April 30, 2016
High-throughput creation of Nicotiana tabacum gene-targeted mutants based on CRISPR/Cas9
Yudong Chen1, Haiying Xiang1, Ling Jia2
1Yunnan Academy of Tobacco Science, Kunming, 650106, China.
Key Message:
4382 available sgRNAs targeting 1060 tobacco genes were obtained, and 10,682 targeted mutants were created using high-throughput methods. Four optimization experiments were established to solve problems encountered during genetic transformation.
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