Related Experiment Video

Updated: Aug 14, 2026

Quantitative Analysis of Chromatin Proteomes in Disease
08:11

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

The distribution of nuclear proteins and transcriptionally-active sequences in rat liver chromatin fractions

N M Mironov, V V Lobanenkov, G H Goodwin

    Experimental Cell Research
    |December 1, 1986
    PubMed

    More Related Videos

    Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis09:26

    Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

    Published on: March 23, 2021

    A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment08:49

    A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment

    Published on: June 5, 2026

    Related Concept Videos

    Inheritance of Chromatin Structures03:17

    Inheritance of Chromatin Structures

    Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
    Euchromatin01:01

    Euchromatin

    The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
    Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
    Chromatin Position Affects Gene Expression02:35

    Chromatin Position Affects Gene Expression

    Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
    Topologically Associated Domains (TADs)
    The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
    Chromatin Structure Regulates pre-mRNA Processing02:41

    Chromatin Structure Regulates pre-mRNA Processing

    In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
    The chromatin structure, especially...
    Chromatin Immunoprecipitation- ChIP02:36

    Chromatin Immunoprecipitation- ChIP

    Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
    Types of ChIP
    ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
    Heterochromatin02:38

    Heterochromatin

    The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
    Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...