Related Experiment Video
Updated: Jul 16, 2025
05:55
Author Spotlight: Developing a Rat Model for Weight-Bearing Intervention to Investigate Osteonecrosis of the Femoral Head
Published on: September 27, 2024
579
Blocking CCN2 Reduces Established Bone Loss Induced by Prolonged Intense Loading by Increasing Osteoblast Activity in
Alex G Lambi1, Michele Y Harris2, Mamta Amin2
1Department of Orthopedics and Rehabilitation University of New Mexico Albuquerque NM USA.
JBMR Plus
|September 13, 2023
Summary
High-repetition, high-force tasks cause detrimental bone remodeling. Treatment with anti-CCN2 during rest improved bone density and formation, suggesting its therapeutic potential for overuse-induced bone loss.
Area of Science:
- Bone Biology and Remodeling
- Skeletal Physiology
- Biomedical Research
Background:
- Repetitive high-force tasks can lead to detrimental bone remodeling, specifically osteopenia, rather than beneficial adaptation.
- Understanding the mechanisms behind this bone loss is crucial for developing effective interventions.
Purpose of the Study:
- To investigate the therapeutic potential of anti-CCN2 (FG-3019) in mitigating bone loss induced by a long-term high-repetition, high-force (HRHF) task.
- To compare the effects of anti-CCN2 treatment during a rest period with a control IgG and no treatment.
Main Methods:
- Adult female Sprague-Dawley rats underwent an 18-week HRHF task, inducing radial trabecular bone osteopenia.
- Post-task, rats were rested for 6 weeks with concurrent treatment of either anti-CCN2 or control IgG.
- Microcomputed tomography (MicroCT) and histomorphometry were used to analyze bone structure, osteoblast and osteoclast activity, and mineralization.
Main Results:
- HRHF task resulted in decreased trabecular bone volume fraction and thickness.
- Anti-CCN2 treatment during rest significantly improved bone volume fraction, thickness, osteoblast numbers, mineralization, and bone formation rate compared to untreated and IgG groups.
- Both treatments improved osteoid volume and reduced osteoclast numbers, but anti-CCN2 showed superior effects on bone formation markers.
Conclusions:
- Long-term high-repetition, high-force tasks induce negative bone remodeling and osteopenia.
- Anti-CCN2 treatment during a recovery period effectively reverses task-induced bone loss and promotes bone formation.
- Anti-CCN2 is a promising therapeutic agent for treating bone loss associated with chronic overuse injuries.
Related Concept Videos
01:31Osteoclasts in Bone Remodeling
3.0K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.0K
01:40Bone Remodeling
38.4K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.4K
01:17Hormones and Bone Tissue
2.7K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.7K
01:29Bone Disorders
3.6K
Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
3.6K
01:25Role of Vitamins in Maintaining Bone Health
3.4K
The growth and maintenance of bone are regulated by a combination of nutritional factors, including vitamins, such as vitamin A, B12, C, D, and K.
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
3.4K