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Proofreading

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Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore, it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...

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Related Experiment Video

Updated: Jul 16, 2025

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

Corrigendum: Improved single-cell genome amplification by a high-efficiency phi29 DNA polymerase.

Jia Zhang^1,2,3,4, Xiaolu Su^1,2,3,4, Yefei Wang^2,3,4,5

¹Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China.

Frontiers in Bioengineering and Biotechnology

|September 13, 2023

Summary

This study corrects a previous article DOI. The corrected DOI is 10.3389/fbioe.2023.1233856.

Keywords:

GB1 fusion protein disulfide bond phi29 DNA polymerase process engineering high-efficiency phi29 DNA polymerase single-cell genome amplification

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Last Updated: Jul 16, 2025

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

Detection of Copy Number Alterations Using Single Cell Sequencing

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

Area of Science:

Biomedical Engineering
Scientific Publishing

Context:

Correction of a previously published article DOI.
Ensuring accurate citation and discoverability of scientific literature.

Purpose:

To provide the correct Digital Object Identifier (DOI) for a specific publication.
To rectify an error in the article's metadata.

Summary:

The article DOI has been updated to 10.3389/fbioe.2023.1233856.
This correction ensures proper referencing and access to the research.

Impact:

Improved accuracy in scientific record-keeping.
Facilitates correct citation and retrieval of the research article.