SEMA3B-AS1 suppresses colorectal carcinoma progression by inhibiting Semaphorin 3B-dependent VEGF signaling pathway

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Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma
07:59

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

Published on: September 3, 2021

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Yi-Qing Wang1,2, Hui Chen1,2, Shuang Xu1,2

  • 1Department of Pathology Nanfang Hospital Southern Medical University Guangzhou Guangdong China.

Medcomm
|September 13, 2023
PubMed

Insights

Long noncoding RNA SEMA3B-AS1 is downregulated in colorectal cancer (CRC), suppressing tumor growth and metastasis. This lncRNA holds potential as a diagnostic marker and therapeutic target for CRC.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) play critical roles in cancer development.
  • LncRNA SEMA3B-AS1 is dysregulated in some cancers, but its role in colorectal cancer (CRC) is unknown.

Purpose of the Study:

  • To investigate the function and mechanism of lncRNA SEMA3B-AS1 in colorectal cancer (CRC) progression.

Main Methods:

  • Analysis of SEMA3B-AS1 expression in CRC tissues and cell lines.
  • In vitro and in vivo experiments to assess the effects of SEMA3B-AS1 overexpression on CRC cell growth and metastasis.
  • Mechanistic studies involving gene expression, protein interactions, and epigenetic modifications (H3K9 acetylation).
  • Investigation of the SEMA3B-NRP1 axis and its role in CRC angiogenesis.

Main Results:

  • SEMA3B-AS1 expression was significantly downregulated in CRC tissues and cell lines.
  • Lower SEMA3B-AS1 levels correlated with poor patient survival.
  • Overexpression of SEMA3B-AS1 inhibited CRC cell proliferation and metastasis.
  • SEMA3B-AS1 epigenetically upregulated its sense gene SEMA3B by recruiting EP300.
  • SEMA3B-AS1 suppressed CRC angiogenesis via the SEMA3B-NRP1 axis and vascular endothelial growth factor signaling.

Conclusions:

  • SEMA3B-AS1 acts as a tumor suppressor in colorectal cancer.
  • SEMA3B-AS1 inhibits CRC progression by regulating SEMA3B expression and angiogenesis.
  • SEMA3B-AS1 represents a potential diagnostic biomarker and therapeutic target for CRC.
Keywords:
SEMA3BSEMA3B‐AS1colorectal carcinomaepigenetic regulationtumor angiogenesis

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