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Updated: Jul 16, 2025
A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes
Published on: December 6, 2014
Detection and quantitative analysis of tumor-associated tertiary lymphoid structures
Man Yang1,2, Yurou Che1,2, Kezhen Li1,3
1Department of Radiation Oncology, Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Affiliated Cancer Hospital of University of Electronic Science and Technology of China, Chengdu 610000, China.
Tumor-associated tertiary lymphoid structures (TLSs) are key indicators of immunotherapy response. This review highlights current detection methods and addresses the need for standardized quantitative analysis for improved clinical application.
Area of Science:
- Immunology
- Oncology
- Pathology
Background:
- Tumor-associated tertiary lymphoid structures (TLSs) are aggregates of immune cells within tumors.
- TLS presence correlates with better patient response to immunotherapy and improved prognosis.
- Understanding TLS formation and function is crucial for clinical applications.
Purpose of the Study:
- To review current research on TLS detection and quantification in tumors.
- To identify limitations in existing TLS assessment methods.
- To propose future directions for standardized TLS analysis.
Main Methods:
- Review of existing literature on TLS detection techniques.
- Analysis of qualitative and quantitative methods for TLS assessment.
- Discussion of established methods like H&E, mIHC, mIF, and gene signatures.
Main Results:
- Current detection methods for TLSs include H&E, mIHC, mIF, and gene signatures.
- Standardized quantitative analysis methods for TLSs are currently lacking.
- Key quantitative aspects needing standardization include cell composition, spatial distribution, and maturity.
Conclusions:
- Standardized quantitative assessment of TLSs is essential for their clinical utility.
- Further research is needed to develop and validate quantitative methods for TLS analysis.
- Advancements in TLS quantification will enhance their role in predicting immunotherapy outcomes.
