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Updated: Jul 16, 2025

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
Regulatory response to a hybrid ancestral nitrogenase in Azotobacter vinelandii
Alex J Rivier1, Kevin S Myers2, Amanda K Garcia1
1Department of Bacteriology, University of Wisconsin-Madison , Madison, Wisconsin, USA.
Importance:
Azotobacter vinelandii is a key model bacterium for the study of biological nitrogen fixation, an important metabolic process catalyzed by nitrogenase enzymes. Here, we demonstrate that compatibilities between engineered A. vinelandii strains and nitrogenase variants can be modulated at the regulatory level. The engineered strain studied here responds by adjusting the expression of proteins involved in cellular processes adjacent to nitrogen fixation, rather than that of nitrogenase proteins themselves. These insights can inform future strategies to transfer nitrogenase variants to non-native hosts.
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