Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

1.1K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.1K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

801
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
801
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

7.5K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.5K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-omic profiling of human antibody-secreting cells reveals diverse subsets sustain durable humoral immunity.

bioRxiv : the preprint server for biology·2026
Same author

Development and function of specified thymic iNKT1 cells critically depend on an Ets1-Tbet transcription factor axis.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Reprogramming SREBP1-dependent lipogenesis and inflammation in high-risk breast with licochalcone A: A novel path to cancer prevention.

International journal of cancer·2025
Same author

B-lineage commitment is dependent on a reversible epigenetic switch.

Genes & development·2025
Same author

Schlafen 12 Modulation and Targeting in Acute Myeloid Leukemia.

Cancer research communications·2025
Same author

Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems.

Nature communications·2025

Related Experiment Video

Updated: Jul 16, 2025

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms
09:41

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms

Published on: July 26, 2024

826

TGF-β Promotes the Postselection Thymic Development and Peripheral Function of IFN-γ-Producing Invariant NKT cells.

Roxroy C Morgan1, Cameron Frank2, Munmun Greger2,3

  • 1Committee on Genetics, Genomics and Systems Biology, The University of Chicago, Chicago, IL.

Journal of Immunology (Baltimore, Md. : 1950)
|September 13, 2023
PubMed
Summary
This summary is machine-generated.

Transforming growth factor-beta (TGF-β) signaling is crucial for the development and maintenance of a subset of invariant natural killer T (iNKT1) cells in the thymus. This pathway influences their gene expression and adhesion molecule presentation, highlighting tissue-specific iNKT1 cell regulation.

More Related Videos

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

52.2K
Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice
07:07

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

Published on: June 27, 2020

5.3K

Related Experiment Videos

Last Updated: Jul 16, 2025

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms
09:41

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms

Published on: July 26, 2024

826
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

52.2K
Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice
07:07

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

Published on: June 27, 2020

5.3K

Area of Science:

  • Immunology
  • Cell Biology
  • Developmental Biology

Background:

  • Invariant natural killer T (iNKT) cells are innate-like lymphocytes involved in host defense.
  • The specific roles of thymic iNKT cells in immune regulation are not fully understood.
  • Subsets of thymic iNKT cells influence various immune cell populations and thymic epithelial cells.

Purpose of the Study:

  • To investigate the role of transforming growth factor-beta (TGF-β) signaling in the development and function of thymic invariant NKT1 (iNKT1) cells.
  • To identify specific iNKT1 cell subsets that depend on TGF-β for their development and gene expression.
  • To compare the TGF-β responsiveness of thymic iNKT1 cells with those in peripheral tissues.

Main Methods:

  • Analysis of gene expression profiles in murine thymic iNKT1 cells.
  • Assessment of cell surface receptor expression, including CD49a, CD103, and CD69.
  • Comparison of TGF-β signaling pathways in iNKT1 cells from thymus, liver, and spleen.

Main Results:

  • A subset of murine thymic iNKT1 cells requires TGF-β signaling for postselection development and maintenance of specific genes.
  • TGF-β signaling is essential for the expression of adhesion receptors CD49a and CD103 on these iNKT1 cells, but not CD69.
  • CD244+ c2 thymic iNKT1 cells, known for IFN-γ production and NK-like characteristics, are part of this TGF-β-responsive population.
  • Liver and spleen iNKT1 cells lack the specific TGF-β gene signature found in thymic iNKT1 cells, although TGF-β still influences their phenotype and function.

Conclusions:

  • TGF-β signaling plays a critical role in the development and maintenance of a specific subset of thymic iNKT1 cells.
  • This study reveals heterogeneity in iNKT1 cell development mechanisms across different tissues.
  • A close association exists between a subset of thymic iNKT1 cells and TGF-β-producing cells, supporting their development.