Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K
CRISPR01:59

CRISPR

52.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.2K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

48
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
48
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

52
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
52
Experimental RNAi02:15

Experimental RNAi

6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
RNA Interference01:23

RNA Interference

26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification and structure determination of a type III-Bv CRISPR complex that post-translationally modifies an associated toxin.

Structure (London, England : 1993)·2026
Same author

Integrating mass spectrometry with Nanopore direct RNA sequencing for <i>de novo</i> modification profiling of bacteriophage MS2.

bioRxiv : the preprint server for biology·2026
Same author

A census of anti-CRISPR proteins reveals AcrIE9 and AcrIE13 as inhibitors of the <i>Escherichia coli</i> K12 type IE CRISPR-Cas system.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Bacterial Schlafen proteins mediate phage defence.

Nature microbiology·2026
Same author

A prophage-encoded sRNA limits phage infection of adherent-invasive E. coli.

PLoS pathogens·2026
Same author

Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci.

Structure (London, England : 1993)·2025

Related Experiment Video

Updated: Jul 16, 2025

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
11:28

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Published on: May 23, 2016

17.7K

CRISPR-based engineering of RNA viruses.

Artem Nemudryi1, Anna Nemudraia1, Joseph E Nichols1

  • 1Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.

Science Advances
|September 13, 2023
PubMed
Summary

Scientists developed a new CRISPR-based RNA editing technology. This method allows precise RNA deletions and insertions, offering new tools for RNA virus engineering.

More Related Videos

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
09:16

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

11.3K
Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.4K

Related Experiment Videos

Last Updated: Jul 16, 2025

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
11:28

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Published on: May 23, 2016

17.7K
A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
09:16

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Published on: October 30, 2016

11.3K
Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.4K

Area of Science:

  • Molecular Biology
  • Genetic Engineering
  • Virology

Background:

  • CRISPR RNA-guided endonucleases offer precise DNA editing capabilities.
  • Current methods for direct RNA editing are limited, hindering research and therapeutic applications.

Purpose of the Study:

  • To develop a novel technology for precise RNA editing.
  • To enable targeted deletions and insertions within RNA molecules.
  • To establish a versatile platform for RNA virus engineering.

Main Methods:

  • Combined CRISPR-Cas ribonucleases for sequence-specific RNA cleavage.
  • Integrated programmable RNA repair mechanisms.
  • Developed a recombinant RNA technology framework.

Main Results:

  • Demonstrated precise RNA deletions and insertions using the developed system.
  • Established a facile method for engineering RNA.

Conclusions:

  • The novel CRISPR-based RNA editing technology provides precise control over RNA sequences.
  • This platform has immediate applications in the engineering of RNA viruses, advancing molecular biology and virology research.