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Area of Science:

  • Biochemistry
  • Toxicology
  • Analytical Chemistry

Background:

  • Microcystin-LR (MC-LR) poses significant health risks, but effective tracing methods in biological systems are lacking.
  • Understanding MC-LR toxicity mechanisms is crucial but hindered by the absence of specific detection tools.
  • Near-infrared (NIR) fluorescent probes offer potential for biological imaging but require tailored design strategies for specific analytes.

Purpose of the Study:

  • To develop novel NIR fluorescent probes for specific detection and imaging of MC-LR in biological systems.
  • To address the limitations of existing methods for MC-LR visualization and mechanistic toxicity studies.
  • To create tools that facilitate a deeper understanding of MC-LR's biological impact.

Main Methods:

  • A facile design strategy was employed to construct three novel NIR fluorescent probes: MC-RdTPA1, MC-RdTPA2, and MC-RdTPE1.
  • The probes were characterized for their fluorescence properties, including emission wavelength and Stokes shift.
  • The probes' ability to selectively visualize MC-LR in cells and their performance across a physiological pH range (5.0-7.0) were evaluated.

Main Results:

  • The developed NIR fluorescent probes exhibit long emission wavelengths and large Stokes shifts, suitable for in vivo imaging.
  • These probes demonstrated selective visualization of MC-LR within cellular environments.
  • Stable fluorescence intensity was observed across a pH range of 5.0-7.0, indicating robustness in biological conditions.

Conclusions:

  • A new strategy for designing NIR fluorescent probes for MC-LR detection has been successfully established.
  • The novel probes provide a valuable tool for visualizing MC-LR in biological systems, advancing toxicity studies.
  • This research opens new avenues for MC-LR detection and enhances the understanding of its toxicological mechanisms.