Comprehensive Analysis of Equid Herpesvirus Recombination: An Insight Into the Repeat Regions
Rocío Lucía Tau1, Carola Ferreccio2, Natalia Bachir1
1Institute of Virology and Technological Innovations, IVIT (INTA-CONICET), Dr Nicolas Repetto and De los Reseros, CP 1686, Hurlingham, Buenos Aires, Argentina.
Abstract:
High-throughput sequencing of genomes has expanded our knowledge of the Alphaherpesvirinae, a widely extended subfamily of DNA viruses that recombine to increase their genetic diversity. It has been acknowledged that equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4), two alphaherpesviruses with an economic impact on the horse industry, can recombine. This work aimed to analyze interspecific recombination between all equid alphaherpesvirus species, using genomes of EHV-1, EHV-3, EHV-4, EHV-6, EHV-8, and EHV-9 available in GenBank. 14 events of recombination by RDP4 and Simplot between EHV-1 x EHV-4, EHV-1 x EHV-9, EHV-8 x EHV-1, and EHV-8 x EHV-9 were identified. Ten out of 14 events involved ORF64, a double-copy gene located at the repeat regions that codifies for the infected cell protein 4 (ICP4). Among the ICP4, recombination can be found between EHV-1 X EHV-9, EHV-8 X EHV-9, and EHV-1 X EHV-4, the former affects zebra-borne genotypes, a type of EHV-1 that infect wild equids, and the latter match with previous breakpoints reported in fields isolates. Consequently, these findings strongly suggest that ICP4 is a hotspot for recombination. This work describes novel recombination events and is the first genome-wide recombination analysis using all available equid alphaherpesvirus species genomes.
More Related Videos
09:57Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
Published on: March 17, 2016
05:22Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
Published on: September 13, 2024
Related Concept Videos
Viral Recombination
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Homologous Recombination
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon...
Retroviruses
