Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glycolysis: Preparatory Phase01:21

Glycolysis: Preparatory Phase

13.6K
In cellular metabolism (the complete breakdown of glucose to extract energy),  glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...
13.6K
ATP Energy Storage and Release01:31

ATP Energy Storage and Release

9.9K
ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...
9.9K
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

13.2K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
13.2K
Other Glycolytic Pathways01:24

Other Glycolytic Pathways

38
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
38
Glycolysis: Pay-off Phase01:25

Glycolysis: Pay-off Phase

10.0K
So far, glycolysis has cost the cell two ATP molecules and produced two small, three-carbon sugar molecules. These molecules will proceed through the second half of the pathway, and sufficient energy will be extracted to pay back the two ATP molecules used as an initial investment and produce a profit for the cell of two additional ATP molecules and two even higher-energy NADH molecules.
Step 1 - 5: Glycolysis Preparatory Phase
The first phase of glycolysis has 5 steps where the glucose is...
10.0K
Fates of Pyruvate01:20

Fates of Pyruvate

8.6K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perceptions and experiences of patients with stable coronary heart disease participating in shared decision-making: a qualitative study in China.

BMJ open·2026
Same author

Dietary <i>Lonicera japonica</i> supplementation modulates cecal gut microbial composition and metabolomic profiles in weaned piglets.

Frontiers in veterinary science·2026
Same author

Deployment, dispatch, and delivery: a scoping review of drone-delivered AED for out-of-hospital cardiac arrest.

Frontiers in public health·2026
Same author

Protocol for a single-blind, single-centre, parallel-group, non-inferiority randomised controlled trial: comparison of oxygenation stability between different ventilators in mechanically ventilated patients during intrahospital and interhospital transport.

BMJ open·2026
Same author

Provoking or backfiring? A contingent model of how abusive supervision influences learning from failure through fear.

Frontiers in psychology·2026
Same author

Effects of Leaf Nutrients, Non-Structural Carbohydrates, and Microanatomical Structure on Biomass of Three Tree Species Under Drought Stress.

Biology·2026

Related Experiment Video

Updated: Jul 16, 2025

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
13:38

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

14.3K

PFKP: More than phosphofructokinase.

Haizhen Wang1, Tiffany Penaloza2, Amanda J Manea2

  • 1Department of Cell and Molecular Pharmacology & Experimental Therapeutics, Medical University of South Carolina, Charleston, SC, United States; Hollings Cancer Center, Medical University of South Carolina, Charleston, SC, United States.

Advances in Cancer Research
|September 13, 2023
PubMed
Summary

Phosphofructokinase (PFK) is a key enzyme in glycolysis. This review details PFKP

Keywords:
Cancer therapyImmune cellsPFKPPost-modificationsPrognostic markerSubcellular location

More Related Videos

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

2.5K
Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

8.5K

Related Experiment Videos

Last Updated: Jul 16, 2025

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
13:38

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

14.3K
A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

2.5K
Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

8.5K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Cancer Research

Background:

  • Phosphofructokinase (PFK) is a crucial enzyme in glycolysis.
  • PFKP's roles in cell proliferation, apoptosis, autophagy, migration, and stemness are known.
  • PFKP functions in various cellular compartments, including cytoplasm, cell membrane, mitochondria, lysosomal membrane, and nucleus.

Approach:

  • This review summarizes PFKP regulation in cancer cells.
  • It highlights PFKP's functions in both glycolysis-dependent and independent pathways.
  • The review also notes the limited research on PFKP in immune cells.

Key Points:

  • PFKP is extensively studied in cancer cells, where it is often overexpressed.
  • PFKP's diverse cellular functions suggest its significant role in cancer progression.
  • PFKP's expression and activity are tightly regulated in cancer cells.

Conclusions:

  • PFKP overexpression and significant functions in cancer cells indicate its potential as a prognostic marker.
  • Targeting or inhibiting PFKP presents a promising therapeutic strategy for cancer treatment.
  • Further research into PFKP's role in immune cells is warranted.