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MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
Published on: November 30, 2013
Identification of microRNA editing sites in clear cell renal cell carcinoma
Yulong Liu1, Shiyong Guo2, Wenping Xie2,3
1State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China.
Abstract:
Clear cell renal cell carcinoma (ccRCC) is a malignant tumor originating from the renal tubular epithelium. Although the microRNAs (miRNAs) transcriptome of ccRCC has been extensively studied, the role of miRNAs editing in ccRCC is largely unknown. By analyzing small RNA sequencing profiles of renal tissues of 154 ccRCC patients and 22 normal controls, we identified 1025 miRNA editing sites from 246 pre-miRNAs. There were 122 editing events with significantly different editing levels in ccRCC compared to normal samples, which include two A-to-I editing events in the seed regions of hsa-mir-376a-3p and hsa-mir-376c-3p, respectively, and one C-to-U editing event in the seed region of hsa-mir-29c-3p. After comparing the targets of the original and edited miRNAs, we found that hsa-mir-376a-1_49g, hsa-mir-376c_48g and hsa-mir-29c_59u had many new targets, respectively. Many of these new targets were deregulated in ccRCC, which might be related to the different editing levels of hsa-mir-376a-3p, hsa-mir-376c-3p, hsa-mir-29c-3p in ccRCC compared to normal controls. Our study sheds new light on miRNA editing events and their potential biological functions in ccRCC.
Insights
This study reveals crucial microRNA (miRNA) editing events in clear cell renal cell carcinoma (ccRCC). Altered miRNA editing in ccRCC impacts gene targets, offering new insights into kidney cancer development.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Clear cell renal cell carcinoma (ccRCC) is a kidney cancer with a largely unknown microRNA (miRNA) editing landscape.
- While miRNA transcriptomes are studied, the functional impact of miRNA editing in ccRCC remains poorly understood.
Purpose of the Study:
- To investigate the role and significance of miRNA editing events in ccRCC.
- To identify specific miRNA editing sites and their altered levels in ccRCC compared to normal kidney tissues.
Main Methods:
- Analysis of small RNA sequencing data from 154 ccRCC patients and 22 normal controls.
- Identification and quantification of miRNA editing sites and their differential levels.
Main Results:
- 1025 miRNA editing sites were identified across 246 pre-miRNAs.
- 122 significant editing events were found in ccRCC, including A-to-I editing in hsa-mir-376a-3p and hsa-mir-376c-3p, and C-to-U editing in hsa-mir-29c-3p seed regions.
- Edited miRNAs (hsa-mir-376a-1_49g, hsa-mir-376c_48g, hsa-mir-29c_59u) exhibited new targets, many deregulated in ccRCC.
Conclusions:
- MiRNA editing is a significant factor in ccRCC pathogenesis.
- Differential miRNA editing levels in ccRCC may contribute to the deregulation of novel target genes, impacting kidney cancer progression.
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02:23RNA Editing