Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Neurulation01:30

Neurulation

40.2K
Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the...
40.2K
Alzheimer Disease l: Introduction01:29

Alzheimer Disease l: Introduction

21
Alzheimer disease is a chronic, progressive, and irreversible neurodegenerative disorder and the most common cause of dementia in older adults. It leads to gradual neuronal loss, causing cognitive decline, behavioral changes, and loss of functional independence.Risk Factors and EtiologyThe disease is multifactorial. Age is the strongest risk factor, with prevalence doubling every 5 years after age 65. Genetic factors include mutations in genes such as APP, PSEN1, and PSEN2, which are associated...
21
Dementia l: Introduction01:22

Dementia l: Introduction

35
Dementia is an acquired, progressive syndrome characterized by a decline in multiple cognitive domains severe enough to impair daily functioning and reduce independence. Although memory loss is a central feature, the diagnosis requires additional deficits involving language, executive function, visuospatial skills, judgment, calculation, or abstract reasoning. These cognitive impairments reflect underlying neurodegenerative or vascular processes that gradually disrupt neuronal networks...
35

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of treatment response in recurrent medulloblastoma using craniospinal MRI.

European journal of radiology·2026
Same author

A uniform tissue-clearing framework and mesoSPIM-ultra enable cm-scale single-neuron tracing.

bioRxiv : the preprint server for biology·2026
Same author

Case Report: Vagus nerve stimulation in super-refractory status epilepticus: Delayed seizure control and focal cortical knife-blade atrophy.

Epilepsy & behavior reports·2026
Same author

Mitochondria limit coenzyme Q export under cholesterol biosynthetic stress.

The Journal of cell biology·2026
Same author

Single-cell multi-omic atlas and morphogen screening informs midbrain and hindbrain organoid engineering.

Nature neuroscience·2026
Same author

What you see is not always what you get-MRI-based ganglionic eminence volumetry challenges subjective assessment in CNS anomalies.

European radiology·2026

Related Experiment Video

Updated: May 2, 2026

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders
07:40

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders

Published on: April 14, 2017

20.7K

Single-cell brain organoid screening identifies developmental defects in autism.

Chong Li1, Jonas Simon Fleck2, Catarina Martins-Costa3

  • 1Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna, Austria. chong.li@imba.oeaw.ac.at.

Nature
|September 13, 2023
PubMed
Summary

We developed a CRISPR-based system to screen genes linked to autism spectrum disorder in human brain organoids. This method identified vulnerable cell types and gene networks affected by these mutations.

More Related Videos

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells NPCs
10:47

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells NPCs

Published on: March 2, 2018

10.0K
Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies
05:45

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

Published on: March 29, 2024

2.4K

Related Experiment Videos

Last Updated: May 2, 2026

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders
07:40

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders

Published on: April 14, 2017

20.7K
Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells NPCs
10:47

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells NPCs

Published on: March 2, 2018

10.0K
Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies
05:45

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

Published on: March 29, 2024

2.4K

Area of Science:

  • Neuroscience
  • Genetics
  • Developmental Biology

Background:

  • Human brain development has unique processes that can lead to neurodevelopmental disorders.
  • Cerebral organoids offer a model to study these disorders in a human context.

Purpose of the Study:

  • To develop a high-throughput screening system for identifying genes contributing to neurodevelopmental disorders.
  • To investigate the effects of perturbing autism spectrum disorder-associated genes on human brain organoid development.

Main Methods:

  • Developed the CRISPR-human organoids-single-cell RNA sequencing (CHOOSE) system for pooled loss-of-function screening.
  • Utilized inducible CRISPR-Cas9 for genetic disruption and single-cell transcriptomics in mosaic organoids.
  • Constructed a developmental gene regulatory network from single-cell transcriptomes and chromatin data.

Main Results:

  • Perturbation of 36 high-risk autism spectrum disorder genes revealed impacts on cell fate determination.
  • Dorsal intermediate progenitors, ventral progenitors, and upper-layer excitatory neurons were identified as vulnerable cell types.
  • Disruption of the ARID1B subunit of the BAF chromatin remodeling complex affected progenitor cell fate transitions.

Conclusions:

  • The CHOOSE system enables efficient screening of disease susceptibility genes in organoid models.
  • This study identified critical cell types and gene regulatory networks involved in neurodevelopmental disorders.
  • Findings provide a foundation for high-throughput phenotypic characterization of genes in organoid systems.