Related Experiment Video
Updated: Jul 16, 2025

06:19
Comparison of Three Clinical Stereoscopic Methods for Measuring Binocular Visual Function During Amblyopic Treatment in Unilateral Amblyopia
Published on: September 27, 2024
257
Test retest variability in stereoacuity measurements
Jignasa Mehta1, Anna O'Connor1
1School of Health Sciences, University of Liverpool.
Strabismus
|September 14, 2023
Summary
This study compared the test-retest variability of four stereoacuity tests in adults. The Asteroid test showed the least variation, while TNO and Frisby offered good reliability over a narrower range.
Area of Science:
- Ophthalmology
- Vision Science
- Clinical Measurement
Background:
- Clinician's choice of stereotest relies on measurement robustness, including sensitivity, specificity, and test-retest variability.
- Limited data exist on the test-retest variability of newer stereotests compared to established ones.
- Understanding test-retest variability is crucial for accurate clinical assessment of stereoacuity.
Purpose of the Study:
- To determine the test-retest variability of four stereotests: TNO, Frisby, Lang Stereopad, and Asteroid (Accurate STEReotest On a mobIle Device).
- To compare stereoacuity measurements between these tests in an adult population.
- To provide data for clinicians selecting reliable stereotests.
Main Methods:
- Stereoacuity was measured twice in adult participants (≥18 years) with normal vision using TNO, Frisby, Lang Stereopad, and Asteroid.
- Participants had best-corrected visual acuity ≥0.3 logMAR and interocular difference <0.2 logMAR.
- Bland-Altman analysis assessed agreement within and between tests; Wilcoxon signed-rank tests compared stereo thresholds.
Main Results:
- Fifty-four adults participated. Test-retest variability was clinically insignificant for TNO, Frisby, and Asteroid (mean bias ≤0.06 log seconds of arc).
- The Asteroid test exhibited the smallest variation between repeated measures (mean bias: -0.01 log seconds of arc).
- The Frisby and Lang Stereopad tests showed the narrowest and widest limits of agreement, respectively. Significant mean bias was found between Frisby and Lang Stereopad.
Conclusions:
- TNO and Frisby tests demonstrate good reliability but cover a narrower stereoacuity range.
- The Asteroid test offers less variation on repeated testing and a wider testing range.
- Further research is needed to assess variability in individuals with reduced stereoacuity.

