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Updated: Jul 16, 2025

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
Published on: August 15, 2018
Perspective: Hidden biases in isotope delta results and the need for comprehensive reporting
Philip J H Dunn1, Grzegorz Skrzypek2
1National Measurement Laboratory, LGC Limited, Teddington, UK.
Abstract:
Measurements of stable-isotope composition on an isotope-delta scale can be subject to bias between laboratories or over time within a single laboratory. This bias can arise not just from differences in method protocol but also from changes in reporting guidelines, or even to the isotope-delta scales themselves. Without a clear description of method protocols, including all sample preparation steps, instrumental parameters and settings, data processing including calibration of results and estimation of measurement uncertainty, the traceability and comparability of isotope-delta values cannot be assured as bias(es) may remain hidden. To address this need, there are now clear guidelines published by IUPAC for reporting isotope-delta values for the "light" elements hydrogen, carbon, nitrogen, oxygen and sulfur.1 We recommend that authors and reviewers adhere to those guidelines when preparing and reviewing future publications.
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