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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Related Experiment Video

Updated: Jul 16, 2025

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
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Reed microstructure detection by optical coherence tomography, an efficient and non-invasive method.

Jin Chen, Liang Zhu, Xinyi Gu

    Applied Optics
    |September 14, 2023
    PubMed
    Summary
    This summary is machine-generated.

    Optical coherence tomography (OCT) non-invasively analyzes woodwind instrument reeds, revealing microstructural changes and predicting lifespan. This advanced technique offers real-time insights without damaging the Arundo donax Linn material.

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    Area of Science:

    • Materials Science
    • Acoustics
    • Biomedical Engineering

    Background:

    • Woodwind instrument reeds are typically made from Arundo donax Linn (ADL).
    • ADL's mechanical properties critically impact reed acoustics and instrument performance.
    • Current methods for analyzing reed microstructure are destructive, limiting further study.

    Purpose of the Study:

    • To introduce optical coherence tomography (OCT) as a non-invasive method for characterizing ADL reeds.
    • To investigate the internal microstructure of reeds and its relationship to performance and lifespan.
    • To demonstrate OCT's capability for real-time, 3D defect analysis.

    Main Methods:

    • Utilized optical coherence tomography (OCT) for 2D and 3D imaging of reed microstructure.
    • Analyzed backscattered optical data to identify microstructural variations.
    • Correlated microstructural changes with vibratory load excitation and usage duration.

    Main Results:

    • Observed degradation of vessel wall microstructure and lumen expansion under vibratory load.
    • Found diminished backscattered signal intensity in parenchymal tissue with extended use.
    • Demonstrated OCT's ability to quantify defect volumes in 3D.

    Conclusions:

    • OCT provides a powerful, non-invasive, real-time technique for reed characterization.
    • Microstructural analysis using OCT can predict reed lifespan and identify defects.
    • This method offers significant advantages over traditional destructive techniques.