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Updated: Jul 16, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Simultaneous imaging of CH*, C2∗, and temperature in flames using a DSLR camera and structured illumination
Abstract:
Measurement of chemical species and temperature mapping in flames is essential to understanding the combustion process. Multiple cameras are conventionally employed for measurement in such scenarios making the experimental setup not only cost-intensive but also challenging. To circumvent this, structured illumination (SI)-based methods are reported for multispecies chemiluminescence (CL) imaging using a single camera. In this paper, we demonstrate four-channel SI-based imaging for simultaneous snapshot C H ∗ and C2∗ CL imaging and two-color pyrometry for temperature profiles in a butane diffusion flame. We demonstrate our approach using individual species and multiple species imaging. Taking the advantage of the axisymmetric nature of the flame, the Abel transform is performed on the line-of-sight averaged images to obtain deconvoluted images. The deconvoluted maps of temperature are compared with the temperature data obtained by using a physical thermocouple probe.
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