Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

65
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
65
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.8K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.8K
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

415
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
415
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advances in Digital Holography, 3-D Imaging, and Optical Information Processing: introduction.

Biomedical optics express·2026
Same author

3D-DeepZern: a deep convolutional neural network for tomographic reconstruction based on Zernike polynomials.

Applied optics·2026
Same author

Deep learning caustic image generation.

Applied optics·2026
Same author

Advances in digital holography, 3-D imaging, and optical information processing: introduction.

Applied optics·2026
Same author

Advances in digital holography, 3-D imaging, and optical information processing: introduction.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same author

Digital Holographic Microscopy for Phenotypic Profiling of Adherent Cells.

Methods in molecular biology (Clifton, N.J.)·2026

Related Experiment Video

Updated: Jul 16, 2025

Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure
10:22

Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure

Published on: February 12, 2018

10.7K

Three-dimensional refractive index estimation based on deep-inverse non-interferometric optical diffraction

Brad Bazow, Thuc Phan, Christopher B Raub

    Optics Express
    |September 15, 2023
    PubMed
    Summary

    This study presents new methods for label-free 3D refractive index mapping of biological samples using optical diffraction tomography (ODT). These techniques enable quantitative imaging without complex interferometry, utilizing simplified intensity measurements.

    More Related Videos

    Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
    13:43

    Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

    Published on: June 24, 2013

    14.1K
    Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
    08:51

    Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

    Published on: May 27, 2008

    13.2K

    Related Experiment Videos

    Last Updated: Jul 16, 2025

    Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure
    10:22

    Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure

    Published on: February 12, 2018

    10.7K
    Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
    13:43

    Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

    Published on: June 24, 2013

    14.1K
    Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
    08:51

    Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

    Published on: May 27, 2008

    13.2K

    Area of Science:

    • Biophotonics
    • Optical Imaging
    • Computational Microscopy

    Background:

    • Optical Diffraction Tomography (ODT) is a powerful technique for label-free 3D refractive index (RI) mapping of biological specimens.
    • Traditional ODT often relies on interferometric setups, which can be complex and sensitive to environmental noise.
    • Developing non-interferometric ODT methods is crucial for broader accessibility and application in biological research.

    Purpose of the Study:

    • To demonstrate 3D refractive index (RI) retrieval methods for partially-coherent ODT systems.
    • To enable quantitative RI contrast using simplified, intensity-only measurements.
    • To validate novel ODT approaches for label-free 3D imaging of biological samples.

    Main Methods:

    • Utilizing intensity-only measurements with axial and angular illumination scanning in a partially-coherent ODT system.
    • Implementing a traditional iterative tomographic solver with gradient descent optimization for focus-scanning systems.
    • Employing 3D convolutional neural networks (CNNs) as an alternative approach to invert the optical scattering process.

    Main Results:

    • Successful demonstration of 3D RI retrieval using simplified, non-interferometric ODT techniques.
    • Validation of both iterative and deep learning (CNN-based) methods through simulations.
    • Quantitative RI contrast was achieved for weak phase 3D biological samples.

    Conclusions:

    • Partially-coherent ODT with intensity-only measurements offers a simplified pathway to label-free 3D RI mapping.
    • Both iterative and CNN-based methods show promise for accurate RI reconstruction in biological imaging.
    • These advancements can enhance the study of biological structures without the need for exogenous labels.