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Effect of M.bovis BCG and M.microti on sarcoma 180 growth in mice
Abstract:
The antitumorous efficacy of the Czechoslovak BCG 725, Czechoslovak BCG 901, the Danish SAS maintained, the Japanese SAS and SH maintained BCG strain and the Czechoslovak OV 247 (M.microti) strain was studied. (SAS--original Sauton's medium with asparagine, SH--Sauton's medium with the asparagine replaced by enzymatic casein hydrolysate.) Ascitic Sa 180 cells were used as the experimental tumor and 800 mice as experimental animals. All the strains employed displayed an anticarcinogenic effect in a protective assay (immunization 8 weeks before inoculation of Sa 180 cells). In a therapeutic test (vaccine administered 24 hr after inoculation of Sa 180 cells and then at weekly intervals), Czechoslovak BCG 725 and Czechoslovak OV 247 were used and proved effective.
Insights
Several Bacillus Calmette-Guérin (BCG) strains demonstrated antitumorous effects against Sa 180 cells in mice. Czechoslovak BCG 725 and Czechoslovak OV 247 showed efficacy in therapeutic settings.
Area of Science:
- Immunology
- Oncology
- Microbiology
Background:
- Bacillus Calmette-Guérin (BCG) is a widely used immunotherapy for cancer.
- Different BCG strains and preparation methods may influence efficacy.
- Evaluating diverse BCG strains is crucial for optimizing cancer treatment.
Purpose of the Study:
- To compare the antitumorous efficacy of various Czechoslovak, Danish, and Japanese BCG strains.
- To assess BCG efficacy in both protective (preventive) and therapeutic settings against experimental tumors.
Main Methods:
- The study utilized ascitic Sa 180 cells as an experimental tumor model in 800 mice.
- Various BCG strains, including Czechoslovak BCG 725, Czechoslovak BCG 901, Danish SAS, Japanese SAS, SH maintained BCG, and Czechoslovak OV 247 (M. microti), were tested.
- Protective assays involved immunization 8 weeks before tumor cell inoculation.
- Therapeutic tests administered BCG 24 hours post-inoculation and weekly thereafter.
Main Results:
- All tested BCG strains exhibited anticarcinogenic effects in the protective assay.
- Czechoslovak BCG 725 and Czechoslovak OV 247 demonstrated significant antitumorous efficacy in the therapeutic test.
- SAS (Sauton's medium with asparagine) and SH (Sauton's medium with enzymatic casein hydrolysate) denote different culture media.
Conclusions:
- Multiple BCG strains possess antitumorous properties.
- Czechoslovak BCG 725 and Czechoslovak OV 247 are effective in therapeutic cancer treatment models.
- Strain variation and administration timing significantly impact BCG's anticarcinogenic potential.