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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Jul 16, 2025

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

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HIV infection detection using CRISPR/Cas systems: Present and future prospects.

Bingpeng Deng1,2, Jing Xue1,2,3

  • 1Beijing Key Laboratory for Animal Models of Emerging and Re-Emerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Beijing 100021, China.

Computational and Structural Biotechnology Journal
|September 15, 2023
PubMed
Summary

CRISPR-based assays offer rapid, accurate, and accessible human immunodeficiency virus (HIV) testing. These point-of-care technologies show promise for improved global HIV detection and management.

Keywords:
ApproachesCRISPRHIV detectionPOCPersonal testing

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Infectious Disease Diagnostics

Background:

  • Human immunodeficiency virus (HIV) infection remains a significant global public health concern.
  • Timely and effective HIV testing is crucial for managing the epidemic.
  • CRISPR-based assays offer innovative solutions for pathogen detection.

Purpose of the Study:

  • To review CRISPR/Cas systems and their applications for HIV detection.
  • To explore the potential of CRISPR-based assays as point-of-care (POC) diagnostic tools.
  • To discuss future perspectives for CRISPR-based HIV testing.

Main Methods:

  • Review of CRISPR/Cas systems, focusing on Cas proteins.
  • Analysis of CRISPR applications in HIV detection methodologies.
  • Exploration of emerging trends in nucleic acid amplification-free and genotype-specific testing.

Main Results:

  • CRISPR-based assays provide portability, digitization, low cost, and ease of use.
  • These assays demonstrate rapid, accurate, and accessible pathogen detection capabilities.
  • CRISPR technology holds significant potential for point-of-care (POC) HIV diagnostics.

Conclusions:

  • CRISPR-based assays represent a promising advancement in HIV detection technology.
  • Future developments may include nucleic acid amplification-free testing and improved detection of HIV genotypes and drug resistance.
  • These innovations could enhance global HIV surveillance and patient management strategies.