Related Experiment Video
Updated: Jul 16, 2025
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Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
Published on: April 21, 2016
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Clickable Dynamic Bioinks Enable Post-Printing Modifications of Construct Composition and Mechanical Properties
Pierre Tournier1, Garance Saint-Pé1, Nathan Lagneau1
1RMeS - Regenerative Medicine and Skeleton (INSERM UMR 1229), Oniris, CHU Nantes, INSERM, Nantes Université, Nantes, F-44000, France.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|September 15, 2023
Summary
Researchers developed novel "clickable dynamic bioinks" for advanced bioprinting. These materials allow post-printing adjustments to properties like stiffness and composition, enabling precise tissue engineering and 4D bioprinting applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Current bioprinting biomaterials often require sacrificial baths or non-physiological conditions.
- Printable biomaterials typically lack tunable composition and mechanical properties.
- Addressing these limitations is crucial for advancing bioprinting applications.
Purpose of the Study:
- Introduce a new biomaterial concept: "clickable dynamic bioinks".
- Develop printable, tunable hydrogels for bioprinting.
- Enable post-printing chemical modifications to fine-tune properties.
Main Methods:
- Utilized hyaluronic acid (HA) to create dynamic hydrogels via boronate ester crosslinking.
- Incorporated bioorthogonal click moieties for post-printing functionalization.
- Demonstrated modifications including composition changes, stiffness tuning, and cell adhesion promotion (RGD peptide immobilization).
Main Results:
- Successfully fabricated printable and cytocompatible dynamic hydrogels.
- Achieved post-printing modifications of polymer composition (HA, chondroitin sulfate, gelatin) and stiffness.
- Enhanced cell adhesion through RGD peptide immobilization.
- Demonstrated spatial and temporal control over modifications.
Conclusions:
- Clickable dynamic bioinks offer a versatile platform for advanced bioprinting.
- These materials overcome limitations of current bioinks by allowing post-printing property tuning.
- The technology paves the way for sophisticated 4D bioprinting and regenerative medicine strategies.
