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Photoluminescence: Fluorescence and Phosphorescence01:23

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Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
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Luminescent polypeptides.

Jieyu Bao1, Chuanye Tong1, Mengxuan He1

  • 1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.

Luminescence : the Journal of Biological and Chemical Luminescence
|September 15, 2023
PubMed
Summary
This summary is machine-generated.

Luminescent polypeptides offer powerful tools for visualizing biological processes. Clusteroluminescent polypeptides show promise as next-generation bioluminophores with high efficiency and tunable wavelengths.

Keywords:
clusteroluminescenceluminescencepolypeptidesproteins

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Area of Science:

  • Biochemistry
  • Biomaterials Science
  • Molecular Biology

Background:

  • Polypeptides are versatile biomacromolecules with applications in tissue engineering, immunomodulation, and target binding.
  • Luminescent polypeptides are gaining attention for their utility in visualizing biological processes.

Purpose of the Study:

  • To review and compile information on different types of luminescent polypeptides.
  • To highlight the potential of clusteroluminescent polypeptides as novel bioluminophores.

Main Methods:

  • Compilation of existing research on luminescent polypeptides.
  • Categorization into natural fluorescent proteins, luminophore-bioconjugated polypeptides, and synthesized clusteroluminescent polypeptides.

Main Results:

  • Identified three main classes of luminescent polypeptides.
  • Highlighted clusteroluminescent polypeptides for their high emission efficiency and tunable wavelengths.

Conclusions:

  • Luminescent polypeptides are valuable tools for biological visualization.
  • Clusteroluminescent polypeptides represent a promising new generation of bioluminophores with significant potential.