Robust and bioaffinity-enhanced nanocarrier based immunochromatographic assay with simplified sample preparation for
Siruo Huang1, Jinliang Chang2, Zhenlin Xu1
1Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Abstract:
Sample pretreatment is directly related to the accuracy and reproducibility of test results, simple and controllable sample pretreatment can greatly reduce analysis errors. Herein, MIL-88B-NH2 was synthesized with a regular octahedral rigid framework structure and used as antibody carrier to develop an immunochromatographic assay (ICA) for the detection of sodium pentachlorophenol (PCP) in animal tissues. MIL-88B-NH2 has superior molar absorption coefficient, ultrahigh antibody affinity, and extreme detection environmental tolerance, which were 1.57, 2.5, and 2 times higher than those of traditional colloidal gold, respectively. Therefore, even complex animal tissues can be detected by simple extraction without nitrogen blowing. Ultimately, the cut-off values of this method for pork, chicken and shrimp were 8/5/3 μg/kg, the limits of detection were 1.15/1.28/0.25 μg/kg. The recoveries ranged from 95.5% to 103.0%, with the coefficient of variation from 1.87% to 9.69%. A parallel analysis of 30 samples was confirmed by LC-MS/MS; the results showed a good correlation (R2 > 0.95), indicating the authenticity and reliability of the MIL-88B-NH2-ICA. This work provides a detection strategy with enhanced sensitivity and robustness and simplified sample pretreatment, which has broad application prospects.
