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Updated: Jul 16, 2025

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Published on: September 22, 2011

High-throughput process development from gene cloning to protein production.

Manman Sun^1,2, Alex Xiong Gao³, Xiuxia Liu^1,4,5

¹National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214112, China.

Microbial Cell Factories

|September 15, 2023

Summary

This review explores high-throughput technologies for recombinant protein expression, offering a holistic strategy for robust protein manufacturing from genes to proteins to meet industry demands.

Keywords:

High-throughput culture High-throughput technology Process development Recombinant protein

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A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Published on: September 22, 2011

High-throughput Purification of Affinity-tagged Recombinant Proteins

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Area of Science:

Biotechnology
Molecular Biology
Biochemistry

Background:

Increased demand for rapid and efficient protein production in the post-genomic era.
Expanding protein sequence databases offer a wider range of enzymes for applications.
Industry and research labs face constraints requiring robust recombinant protein manufacturing processes.

Purpose of the Study:

To review high-throughput technologies for recombinant protein expression.
To present a holistic high-throughput process development strategy.
To identify challenges, limitations, and future directions in the field.

Main Methods:

Exploration of existing literature on high-throughput protein expression.
Analysis of strategies for process development from gene to protein.
Discussion of challenges and limitations in current methodologies.

Main Results:

Identification of key high-throughput technologies applicable to protein production.
Presentation of a comprehensive strategy for optimizing recombinant protein manufacturing.
Highlighting areas for improvement and future research.

Conclusions:

High-throughput approaches are crucial for efficient recombinant protein production.
A holistic strategy is needed to overcome challenges in protein manufacturing.
Further research should focus on refining these technologies and strategies.