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Customized Loading of microRNA-126 to Small Extracellular Vesicle-Derived Vehicles Improves Cardiac Function after
Sruti Bheri1, Milton E Brown1, Hyun-Ji Park1,2
1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, United States.
ACS Nano
|September 16, 2023
Summary
Engineered extracellular vesicle-like vehicles (ELVs) loaded with miR-126 show promise for cardiac repair. These synthetic vesicles improve blood vessel formation and heart function after myocardial infarction in rats.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Cardiovascular Research
Background:
- Small extracellular vesicles (sEVs) show potential for cardiac repair but face challenges with inconsistent production and cargo loading.
- Synthetic sEV mimics offer cargo control but lack in vivo stability and have rapid clearance rates.
Purpose of the Study:
- To develop and evaluate engineered extracellular vesicle-like vehicles (ELVs) for targeted delivery of pro-angiogenic miR-126.
- To assess the efficacy of electroporated ELVs carrying miR-126 in promoting cardiac repair in vitro and in vivo.
Main Methods:
- Development of ELVs using an electroporation technique.
- In vitro assessment of ELV efficacy on cardiac endothelial cell tube formation.
- In vivo evaluation of ELV delivery in a rat model of ischemia-reperfusion injury, using echocardiography and histology.
Main Results:
- Electroporated miR-126+ ELVs significantly improved tube formation in cardiac endothelial cell cultures.
- In vivo administration of miR-126+ ELVs enhanced echocardiographic and histological outcomes in rats post-ischemia-reperfusion injury.
Conclusions:
- Electroporated ELVs serve as effective vehicles for delivering specific microRNA cargo, such as miR-126.
- This approach holds significant potential for advancing cell-free cardiac repair strategies after myocardial infarction.
