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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Related Experiment Video

Updated: Jul 16, 2025

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

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Time resolved applications for Cryo-EM; approaches, challenges and future directions.

David P Klebl1, Louie Aspinall2, Stephen P Muench1

  • 1School of Biomedical Sciences, Astbury Centre for Structural Molecular Biology, University of Leeds, UK.

Current Opinion in Structural Biology
|September 16, 2023
PubMed
Summary

Advancements in cryo-electron microscopy (cryo-EM) enable capturing dynamic molecular states. New time-resolved cryo-EM methods are emerging to study transient biological processes with greater detail.

Keywords:
Cryo-EMProtein dynamicsSample preparationTime-resolved

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Area of Science:

  • Structural Biology
  • Biophysics
  • Biochemistry

Background:

  • Cryo-electron microscopy (cryo-EM) has advanced to provide high-resolution static molecular structures.
  • Capturing dynamic or transient molecular conformations has remained a challenge.

Purpose of the Study:

  • To provide an overview of emerging time-resolved cryo-EM approaches.
  • To discuss the strengths, limitations, and challenges of these novel techniques.

Main Methods:

  • Overview of various time-resolved cryo-EM methodologies.
  • Analysis of techniques for trapping non-equilibrium states within millisecond timescales.

Main Results:

  • New approaches allow trapping and analyzing transient molecular states.
  • Identified challenges in achieving higher resolutions and faster time-framing.

Conclusions:

  • Time-resolved cryo-EM is a valuable addition to the cryo-EM toolkit.
  • These methods expand possibilities for both single particle and tomographic studies.