Interactions between humulinone derived from aged hops and protein Z enhance the foamability and foam stability

Foam is one of the important characteristics of beer, including foamability, foam stability and foam texture. Protein Z (PZ) is considered to be an important component of beer foam. In this study, the interaction between PZ and humulinone, a widespread compound in aged hops, and the effect on foam properties of PZ were investigated. The fluorescence spectra showed that the stoichiometric ratio of humulinone to PZ was 4.25 ± 0.48: 1, and the binding constant was (1.64 ± 0.17) × 10⁵ M^-1. MD and FTIR results showed that the main force of interaction between PZ and humulinone was hydrogen bond, and the possible sites were Asn-37, Ser-292, Lys-290 and Pro-395. Moreover, the addition of humulinone greatly reduced the surface tension of PZ solution, and changed the secondary structure of PZ, which is beneficial for the foam stability. Under the influence of humulinone, the foamability, foam stability and foam texture of PZ all increased.