Nonlinear neural patterns are revealed in high frequency functional near infrared spectroscopy analysis

Functional Near Infrared Spectroscopy (fNIRS) is a useful tool for measuring hemoglobin concentration. Linear theory of the hemodynamic response function supports low frequency analysis (<0.2 Hz). However, we hypothesized that nonlinearities, arising from the complex neurovascular interactions sustaining vasomotor tone, may be revealed in higher frequency components of fNIRS signals. To test this hypothesis, we simulated nonlinear hemodynamic models to explore how blood flow autoregulation changes may alter evoked neurovascular signals in high frequencies. Next, we analyzed experimental fNIRS data to compare neural representations between fast (0.2-0.6 Hz) and slow (<0.2 Hz) waves, demonstrating that only nonlinear representations quantified by sample entropy are distinct between these frequency bands. Finally, we performed group-level distance correlation analysis to show that the cortical distribution of activity is independent only in the nonlinear analysis of fast and slow waves. Our study highlights the importance of analyzing nonlinear higher frequency effects seen in fNIRS for a comprehensive analysis of cortical neurovascular activity. Furthermore, it motivates further exploration of the nonlinear dynamics driving regional blood flow and hemoglobin concentrations.