Quantitative determination of androstenone in pig adipose tissue

A simple, cheap and rapid method for the quantitative determination of the boar taint substance, 5 alpha-androst-16-en-3-one, in pig adipose tissue is described. After saponification of the fat the androstenone is extracted, derivatised with o-(pentafluorobenzyl)hydroxylamine hydrochloride in pyridine and analysed by fused-silica open-tubular capillary gas chromatography with electron-capture detection.