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Updated: Jul 16, 2025

NanoDrop Microvolume Quantitation of Nucleic Acids
Published on: November 22, 2010
Gravimetric deposition of microliter drops with radiometric confirmation
Denis E Bergeron1, Richard Essex1, Svetlana Nour1
1National Institute of Standards and Technology, 100 Bureau Dr., Stop 8462, Gaithersburg, MD, 20899-8462, USA.
Abstract:
A manual microliter gravimetric dispensing technique is demonstrated using a micropipettor modified for use with removeable microcapillaries. Liquid scintillation sources were prepared from a well-characterized 241Am reference solution, providing a radiometric check of dispensed masses. Further experiments confirmed controlled dispensing of drops onto gold foils with losses ≤0.34(4) % of the total drop activity. A detailed measurement equation for the weighing technique, including the corrections for evaporation, is presented with a full accounting of associated uncertainties.
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