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An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
Published on: May 7, 2018
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Growth hormone receptor gene disruption.
Edward O List1, Silvana Duran-Ortiz1, Prateek Kulkarni1
1The Edison Biotechnology Institute, and the Heritage College of Osteopathic Medicine, Ohio University, Athens, OH, United States.
Vitamins and Hormones
|September 17, 2023
Summary
Growth hormone receptor (GHR) knockout mice models reveal diverse physiological roles of growth hormone (GH) across multiple tissues. These models advance our understanding of GH action and generate new research hypotheses.
Area of Science:
- Endocrinology
- Physiology
- Genetics
Background:
- Growth hormone (GH) functions are extensively studied using GH receptor (GHR) knockout mouse models.
- Recent research employs tissue-specific or temporal GHR disruption to investigate GH action.
- Thirty-seven distinct GHR knockout mouse lines have been developed to date.
Purpose of the Study:
- To summarize existing GHR knockout mouse lines.
- To provide background on their generation.
- To highlight key physiological outcomes of GHR gene disruption.
Main Methods:
- Creation of 37 distinct GHR knockout mouse lines.
- Targeted disruption in various tissues including fat, liver, muscle, heart, bone, brain, macrophages, intestine, hematopoietic stem cells, and pancreatic beta cells.
- Inducible multi-tissue 'global' disruption at different ages.
Main Results:
- These mouse lines offer unique insights into GH action.
- Physiological outcomes from GHR gene disruption have been documented.
- New hypotheses regarding GH functions in specific tissues have emerged.
Conclusions:
- Collectively, GHR knockout mouse models are invaluable tools for dissecting GH physiology.
- They facilitate the exploration of GH's diverse roles in various organs and systems.
- These models are crucial for advancing our understanding of endocrine signaling and metabolic regulation.
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