Related Experiment Videos
Lymph node primary immunization of mice for the production of polyclonal and monoclonal antibodies
Primary immunization of mice by lymph node injection of bovine serum albumin (BSA) in doses as low as 1 ng followed by a secondary immunization with 1 microgram each for intraperiteonal and intravenous injections was sufficient to elicit the production of circulating antibodies. The lowest efficient dose tested was the injection of 100 ng of BSA in the lymph nodes and booster injections of 1 ng each. This method was extended to the production of monoclonal antibodies using less than 20 micrograms of a 35 kDa polypeptide purified from hamster cells transformed by Herpes simplex type 2 viruses.
Primary immunization of mice by lymph node injection of bovine serum albumin (BSA) in doses as low as 1 ng followed by a secondary immunization with 1 microgram each for intraperiteonal and intravenous injections was sufficient to elicit the production of circulating antibodies. The lowest efficient dose tested was the injection of 100 ng of BSA in the lymph nodes and booster injections of 1 ng each. This method was extended to the production of monoclonal antibodies using less than 20 micrograms of a 35 kDa polypeptide purified from hamster cells transformed by Herpes simplex type 2 viruses.