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Engineering an activatable fluorescent probe for studying ONOO^- in pyroptotic process.

Jiankang Gong¹, Xiaoyu Wang¹, Hai-En Fan¹

¹College of Health Science and Engineering, Hubei University, Wuhan, 430062, China.

|September 18, 2023

Summary

Researchers developed a new probe to detect peroxynitrite (ONOO-) during pyroptosis, a cell death process crucial for cancer treatment. This tool helps understand ONOO-’s role in pyroptosis and screen drugs that trigger it.

Keywords:

Chemotherapy Fluorescent probe Peroxynitrite Pyroptosis Tumor imaging

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Area of Science:

Biomedical Science
Oncology
Cellular Biology

Background:

Pyroptosis is a programmed cell death pathway vital for cancer treatment, but its mechanisms remain largely unknown.
Reactive oxygen species (ROS), particularly peroxynitrite (ONOO-), are linked to pyroptosis and various diseases.
Understanding the ONOO- pyroptosis connection is crucial for advancing cancer therapies.

Purpose of the Study:

To investigate the role of ONOO- in pyroptosis during tumor treatment.
To develop a sensitive probe for detecting ONOO- fluctuations in real-time.
To explore the utility of this probe in evaluating chemotherapeutic drug efficacy.

Main Methods:

Fabrication of a novel near-infrared (NIR) probe, Rd700-PN, for ONOO- detection.
Utilizing Rd700-PN to monitor ONOO- levels during cellular pyroptosis.
Employing Rd700-PN to assess the pyroptosis-inducing capacity of anticancer drugs in vivo.

Main Results:

Rd700-PN successfully detected ONOO- fluctuations during cellular pyroptosis for the first time.
The probe demonstrated the ability to scout the pyroptosis induction by chemotherapeutic drugs in vivo.
This highlights a new method for studying the ONOO- and pyroptosis interplay.

Conclusions:

A novel NIR probe, Rd700-PN, was developed for sensitive and rapid ONOO- detection.
This study establishes a link between ONOO- and pyroptosis in cancer treatment.
Rd700-PN offers a valuable tool for elucidating pyroptosis mechanisms and screening novel antitumor drugs.