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Updated: Jul 16, 2025
Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Published on: March 3, 2010
Engineering an activatable fluorescent probe for studying ONOO- in pyroptotic process
Jiankang Gong1, Xiaoyu Wang1, Hai-En Fan1
1College of Health Science and Engineering, Hubei University, Wuhan, 430062, China.
Abstract:
Pyroptosis, a recently discovered form of programmed cell death, plays a pivotal role in oncological treatment. Howbeit, the mechanisms underlying pyroptosis in tumor treatment remain unclear. Previous research has demonstrated that the occurrence of pyroptosis generally accompanies a surge of reactive oxygen species (ROS) generation, with ONOO- being one of these ROS and closely linked to numerous diseases. Therefore, it is imperative to investigate the potential association between ONOO- and pyroptosis. Herein, a highly sensitive and rapidly responsive near-infrared (NIR) probe, Rd700-PN, is fabricated for exploring unrevealed relationships between ONOO- and pyroptosis. We successfully harness Rd700-PN to detect ONOO- fluctuation during cellular pyroptosis for the first time. Furthermore, the results demonstrate that Rd700-PN can scout the chemotherapeutic drug's induction ability of tumor pyroptosis in vivo. Notably, this study provides an excellent means to shed light on the correlation between ONOO- and pyroptosis and to screen antitumor drugs activating pyroptosis.
Insights
Researchers developed a new probe to detect peroxynitrite (ONOO-) during pyroptosis, a cell death process crucial for cancer treatment. This tool helps understand ONOO-’s role in pyroptosis and screen drugs that trigger it.
Area of Science:
- Biomedical Science
- Oncology
- Cellular Biology
Background:
- Pyroptosis is a programmed cell death pathway vital for cancer treatment, but its mechanisms remain largely unknown.
- Reactive oxygen species (ROS), particularly peroxynitrite (ONOO-), are linked to pyroptosis and various diseases.
- Understanding the ONOO- pyroptosis connection is crucial for advancing cancer therapies.
Purpose of the Study:
- To investigate the role of ONOO- in pyroptosis during tumor treatment.
- To develop a sensitive probe for detecting ONOO- fluctuations in real-time.
- To explore the utility of this probe in evaluating chemotherapeutic drug efficacy.
Main Methods:
- Fabrication of a novel near-infrared (NIR) probe, Rd700-PN, for ONOO- detection.
- Utilizing Rd700-PN to monitor ONOO- levels during cellular pyroptosis.
- Employing Rd700-PN to assess the pyroptosis-inducing capacity of anticancer drugs in vivo.
Main Results:
- Rd700-PN successfully detected ONOO- fluctuations during cellular pyroptosis for the first time.
- The probe demonstrated the ability to scout the pyroptosis induction by chemotherapeutic drugs in vivo.
- This highlights a new method for studying the ONOO- and pyroptosis interplay.
Conclusions:
- A novel NIR probe, Rd700-PN, was developed for sensitive and rapid ONOO- detection.
- This study establishes a link between ONOO- and pyroptosis in cancer treatment.
- Rd700-PN offers a valuable tool for elucidating pyroptosis mechanisms and screening novel antitumor drugs.
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