Estimation of the Mr of isocitrate lyase messenger RNA from Chlorella fusca

The mRNA for the adaptive enzyme isocitrate lyase (ICL) from Chlorella fusca has been identified by fractionation of total poly(A)-containing RNA and in vitro translation followed by immune precipitation. The Mr of ICL mRNA was approximately 8.0 X 10(5), which is in good agreement with a previous estimate obtained by in vivo double-labelling.