Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

722
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
722
NMR and Mass Spectroscopy of Carboxylic Acids01:30

NMR and Mass Spectroscopy of Carboxylic Acids

4.0K
In ¹H NMR spectroscopy, acidic protons (–COOH) of carboxylic acids are highly deshielded and absorb far downfield, at around 9–12 ppm. The chemical shift value depends on the concentration and solvent used.
While α protons of carboxylic acids absorb at 2–2.5 ppm, β protons absorb further upfield.
Carboxylic acids are easily identified by dissolving them in deuterium oxide, which results in a rapid exchange of the acidic protons with deuterium. This leads to the...
4.0K
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

1.1K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
1.1K
Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

37
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
37

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perturbing glycosylphosphatidylinositol (GPI)-anchor biosynthesis alters cell wall architecture and modulates fungal morphology.

bioRxiv : the preprint server for biology·2026
Same author

Mycoelectronics: Bioprinted living fungal bioelectronics for artificial sensation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Dynamic Reprogramming of Fungal Cell Walls Underlies Germination and Immune Exposure in Zygomycetous Fungal Pathogens.

bioRxiv : the preprint server for biology·2026
Same author

Impact of Prior Conization on Oncological Outcomes of Laparoscopic versus Open Radical Hysterectomy in Early-Stage Cervical Cancer: A Propensity Score-Weighted Retrospective Observational Study.

Journal of minimally invasive gynecology·2026
Same author

Chaotropic ions reshape the cell wall of the obligate halophile aspergillus atacamensis: Insights from solid-state NMR.

Carbohydrate polymers·2026
Same author

α-1,3-Glucan-Driven Remodeling of the Conidial Cell Wall in an <i>Aspergillus fumigatus</i> Vaccine Strain Alters Innate Immune Recognition.

Journal of the American Chemical Society·2026

Related Experiment Video

Updated: Jul 16, 2025

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

7.5K

Charting the solid-state NMR signals of polysaccharides: A database-driven roadmap.

Wancheng Zhao1, Debkumar Debnath1, Isha Gautam1

  • 1Department of Chemistry, Michigan State University, East Lansing, Michigan, USA.

Magnetic Resonance in Chemistry : MRC
|September 19, 2023
PubMed
Summary

This study introduces a statistical method for analyzing solid-state nuclear magnetic resonance (ssNMR) spectra of cell walls. This approach aids in identifying plant and fungal polysaccharides, improving carbohydrate analysis.

Keywords:
13Ccarbohydratecell wallcellulosechitinglucanhemicellulosepectinsolid‐state NMRxylan

More Related Videos

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.5K
Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition
07:40

Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition

Published on: May 17, 2024

1.3K

Related Experiment Videos

Last Updated: Jul 16, 2025

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

7.5K
Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.5K
Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition
07:40

Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition

Published on: May 17, 2024

1.3K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Solid-state nuclear magnetic resonance (ssNMR) of intact cell walls is challenging due to complex mixtures of glycans and structural variations.
  • Interpreting ssNMR spectra of biological samples requires advanced analytical techniques to resolve overlapping signals from various carbohydrates.

Purpose of the Study:

  • To develop and present a statistical approach for analyzing carbohydrate signals in high-resolution ssNMR data.
  • To facilitate the identification of key plant and fungal polysaccharides using a curated carbohydrate database.
  • To address the challenge of spectral overlap and structural polymorphism in ssNMR analysis of cell walls.

Main Methods:

  • Utilized high-resolution solid-state nuclear magnetic resonance (ssNMR) data.
  • Developed a statistical analysis framework for carbohydrate signals.
  • Generated simulated spectra to assess chemical shift dispersion.
  • Compared simulated data with experimental results from fungal and plant samples.
  • Indexed data within a comprehensive carbohydrate database.

Main Results:

  • Successfully demonstrated a method for differentiating characteristic signals of major carbohydrates.
  • Identified important fungal polysaccharides like chitin and glucans.
  • Identified key plant polysaccharides including cellulose, hemicellulose, and pectic polymers.
  • Highlighted that distinct carbohydrates can yield similar ssNMR signals, emphasizing the need for high-resolution data and validated assignments.
  • Provided a summary of currently undetected polysaccharides in plants and fungi.

Conclusions:

  • The developed statistical approach enhances the interpretation of complex ssNMR spectra from cell walls.
  • This method aids in the identification and characterization of diverse polysaccharides in biological samples.
  • The findings pave the way for future investigations into previously undetected polysaccharides in plants and fungi.