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Single bacteria identification with second-harmonic generation in MoS2.

Young Chul Kim1, Seung Won Jun1, Yeong Hwan Ahn1

  • 1Department of Physics and Department of Energy Systems Research, Ajou University, Suwon 16499, South Korea.

Biosensors & Bioelectronics
|September 19, 2023
PubMed
Summary
This summary is machine-generated.

Monolayer molybdenum disulfide (MoS2) exhibits controllable second-harmonic generation (SHG) influenced by bacteria. This SHG imaging enables label-free detection and identification of single bacterial cells, including pathogens.

Keywords:
BacteriaLabel-free sensingMicrobial strainSecond harmonic generationTransition metal dichalcogenides

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biophotonics

Background:

  • Transition-metal dichalcogenides, like monolayer MoS2, possess significant nonlinear optical properties.
  • These properties make them attractive for photonic devices and sensing applications.
  • Microbial interactions with 2D materials are an emerging area of research.

Purpose of the Study:

  • To investigate the impact of single bacteria on second-harmonic generation (SHG) in monolayer MoS2.
  • To explore SHG imaging for label-free bacterial detection, identification, and stress monitoring.
  • To demonstrate UV-induced bacterial removal and characterization using SHG.

Main Methods:

  • Utilizing monolayer MoS2 as a substrate for bacterial deposition.
  • Analyzing anisotropic polarization responses in SHG signals induced by bacteria.
  • Employing SHG imaging to monitor biomaterial strain and bacterial rupture.
  • Investigating UV-induced bacterial removal and species-specific rupture time analysis.

Main Results:

  • Bacteria deposited on MoS2 alter SHG signals, showing anisotropic polarization dependent on bacterial orientation.
  • SHG imaging effectively detects biomaterial strain as low as 0.1% due to bacterial presence.
  • Transient SHG signals reveal species-specific bacterial rupture times after UV exposure.
  • Label-free identification of single bacterial cells, including *E. coli* and *L. casei*, is achieved.

Conclusions:

  • Monolayer MoS2 can be used to monitor bacterial-induced strain and facilitate label-free pathogen identification.
  • SHG imaging offers a sensitive method for single-cell bacterial analysis and differentiation.
  • This approach holds promise for developing advanced diagnostic tools for microbial detection.