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Updated: Jul 16, 2025

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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
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A tolane-modified 5-ethynyluridine as a universal and fluorogenic photochemical DNA crosslinker
Hermann Neitz1, Claudia Höbartner1,2
1Institute of Organic Chemistry, University of Würzburg, Am Hubland, 97074 Würzburg, Germany. claudia.hoebartner@uni-wuerzburg.de.
Abstract:
We report the fluorescent nucleoside ToldU and its application as a photoresponsive crosslinker in three different DNA architectures with enhanced fluorescence emission of the crosslinked products. The fluorogenic ToldU crosslinking reaction enables the assembly of DNA polymers in a hybridization chain reaction for the concentration-dependent detection of a specific DNA sequence.

