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Association between microRNA expression and risk of male idiopathic infertility in Iraq

Manal Mohammed Khadhim1, Abbas Ali Manshd2

  • 1Al-Qadisiyah University, College of Medicine, Department of Medical Microbiology - Diwaniya, Iraq.

Revista Da Associacao Medica Brasileira (1992)
|September 20, 2023
PubMed
Abstract

Insights

Two microRNAs (miRNAs), miR-122 and miR-34c-5p, show potential as biomarkers for male infertility. Their expression levels differ significantly in men with infertility, suggesting diagnostic value.

Area of Science:

  • Reproductive Biology
  • Molecular Genetics
  • Biomarker Discovery

Background:

  • Infertility affects millions globally, with male factors contributing significantly.
  • Idiopathic infertility lacks a clear cause, necessitating novel diagnostic approaches.
  • MicroRNAs (miRNAs) are emerging as key regulators in reproductive health.

Purpose of the Study:

  • To quantify gene expression levels of miR-122 and miR-34c-5p in males with idiopathic infertility.
  • To assess the potential of these miRNAs as predictive and diagnostic biomarkers for male infertility.

Main Methods:

  • A case-control study involving 50 males with idiopathic infertility and 50 healthy controls.
  • Analysis of miR-122 and miR-34c-5p expression levels using quantitative methods.
  • Statistical analysis to determine significant differences between groups.

Main Results:

  • miR-122 levels were highest in azoospermia, followed by oligospermia.
  • Significant differences in miR-34c-5p levels were observed between infertile patients and controls (p<0.05).

Conclusions:

  • miR-122 and miR-34c-5p demonstrate potential as reliable biomarkers for male infertility.
  • These miRNAs could aid in the diagnosis and prediction of infertility in males.
  • Further research is warranted to validate their clinical utility.

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